Effect of Penicillin on Vaccine Virus and Its Value in Purification of Vaccine Lymph

Numerous methods have been tried for reducing the bacterial count of calf lymph without diminishing its potency. Gins (1924) and Lehmann (1937) have used carbolic acid as an agent for the destruction of bacteria in lymph. Stevenson and Butler (1936) drew attention to the value of phenol as an agent for preventing accidental contamination of lymph. They found that lymph made up into 50 per cent glycerol containing 0.5 per cent phenol retains its potency better than lymph made up in 50 per cent glycerol containing 0.1 per cent clove oil. The method followed at this Institute is to prepare lymph in 50 per cent glycerine solution made slightly alkaline by adding sodium bicarbonate as follows :?